Expression of K99 adhesion antigen controlled by the Escherichia coli tryptophan operon promoter
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چکیده
منابع مشابه
Cloning and expression of rhl AB operon under the control of tac promoter in E. coli
Today, efforts go towards the replacement of chemical surfactants by natural biological biosurfactants (biosurfactant), as these materials are not carcinogenic and highly compatibile with the environment. One of the main classes of biosurfactants is rhamnose containing glycolipid biosurfactant (rhamnolipids). This type of biosurfactants can be applied in many industries such as oil industry, ph...
متن کاملRegulation of tryptophan operon expression by attenuation in cell-free extracts of Escherichia coli.
Expression of the tryptophan (trp) operon of Escherichia coli was shown to be regulated by attenuation in an in vitro DNA-dependent protein-synthesizing system. In extracts prepared from a temperature-sensitive tryptophanyl-tRNA synthetase mutant, plasmid-directed trpE enzyme synthesis was inhibited 2- to 3-fold by addition of purified wild type tryptophanyl-tRNA synthetase. When the extract us...
متن کاملTranslational coupling during expression of the tryptophan operon of Escherichia coli.
E. coli trpE polar mutations are 10 time more polar on trpD gene expression than on downstream (trpC, B, or A) gene expression. This effects was shown to be the result of "translational coupling," in which efficient translation of trpE-trpD intercistronic punctuation region consists of overlapping stop and start codons, and the trpE and trpD gene products form a functional complex in the cell. ...
متن کاملRegulation of basal level expression of the tryptophan operon of Escherichia coli.
Mutations in prfB, encoding release factor 2 (UGA- and UAA-specific), increase transcription termination at the trp operon attenuator in Escherichia coli strains grown in the presence of tryptophan. The prfB mutations have no effect on basal level expression in strains in which the natural trp leader peptide stop codon UGA was replaced by either UAG or UAA. The effect of introducing prfB mutati...
متن کاملGlycoprotein glycans that inhibit adhesion of Escherichia coli mediated by K99 fimbriae: treatment of experimental colibacillosis.
Calf diarrhea due to infection by enterotoxigenic Escherichia coli was treated by administration of glycoprotein glycans derived from bovine plasma. The glycan moieties of the nonimmunoglobulin fraction of plasma mimicked the oligosaccharide moiety of intestinal receptors recognized by K99 pili. These glycoprotein glycans inhibited adhesion of E. coli K99+ ST+ to erythrocyte glycoconjugates in ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1988
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.56.9.2317-2323.1988